This research was conducted to determine the alcohol concentration, alcohol immersion time and the ratio of raw materials /solvent to separate the highest amount of wax in rice bran before extracting rice bran oil. The treated rice bran was two processes. Process 1: rice bran – steamed – pickled ethanol – dry - extracted with hexane. Process 2: rice bran - pickled ethanol – dry - extracted with hexane. The treated rice bran was pickled ethanol in different ethanol 96, 90, 80, 70, 60, 50 and 40 respectively with raw materials/ solvent ratio of 1:5 (w/v). Rice bran was investigated for soaking time 15 minutes, 30 minutes, 1 hour, 2 hours, 3 hours respectively. After that, To select the appropriate alcohol content and soaking time were to investigate the ratio of raw materials/solvent 1:1 (w/v), 1:2 (w/v), 1:3 (w/v), 1:4 (w/v), 1:5 (w/v), 1:6 (w/v) and 1:7 (w/v) respectively. The results showed that the best soaking time to separate wax in rice bran was 3 hours with the ratio of raw materials/solvent 1:6 (w/v).
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