Silver nanoplates (AgNPls) are attractive due to their unique properties, including localized surface plasmon res and antibacterial ability due to their vicinity tips and edges. This study reports a rapid and facile methodology for preparing Ag nanoplates using a single-step synthesis approach with the presence of AgNO3 precursor, NaBH4, TSC, and an oxidation agent H2O2. Adjusting precursor ratios can yield various morphologies such as spherical, nanodisks, hexagonal structures, and triangular nanoplates, each exhibiting different LSPR. The optical properties of AgNPs were analyzed using UV-Vis spectroscopy and supported by SEM morphology analysis. The crystal strutures were characterized by TEM, and HR-TEM. Furthermore, the anti-bacterial activity was also evaluated against four bacterial strains, including Staphylococcus aureus, Listeria innocua, Escherichia coli, and Pseudomonas aeruginosa. The AgNPLs prepared in this study exhibited better antibacterial activity against Gram-positive bacteria at low concentrations (0.625 µg/mL) than Gram-negative bacteria.